.. _gettingstarted-home: ############### Getting Started ############### In order to be able to control stage positions (which are used for focus and sample positioning), toggle lasers, trigger cameras, and so on, you first need to set up a connection to your hardware. This guide will walk you through the steps necessary to get your system up and running. Hardware Setup ______________________ Unlike other microscopy systems, Altair simplifies control by having only one controller - one apparatus to trigger and drive all necessary devices. This is Applied Scientific Instrumentation's Tiger Controller, a modular controller with customizable control cards. Our solution calls for a tiger controller with the following cards: - TGCOM - allows Joystick control and serial communication from computer - 3 Stage control cards - (X/Y, Z/F, M/N) - 2 DAC4 cards - Analog signal generators - TGPLC - Programmable Logic Controller which provides for custom timing - FW-1000 - Filter Wheel Control The order of these cards within the controller does not matter. The software to run the controller is `navigate `_. Navigate supports many microscope configurations, where each configuration is defined by a configuration file. The provided configuration file will delineate the following configuration: ========= ============================ **Axis** **Device** ========= ============================ **A** Remote Focus Voice Coil **B** Galvanometer **H** 405 nm Laser (Analog) **I** 488 nm Laser (Analog) **J** 561 nm Laser (Analog) **K** 638 nm Laser (Analog) **1** Camera Trigger **2** Laser Shutter **3** Stage In **4** Stage Out **5** 405 nm Laser (Digital) **6** 488 nm Laser (Digital) **7** 561 nm Laser (Digital) **8** 638 nm Laser (Digital) ========= ============================ The stage control card specified for PLC outputs 3 and 4 is the stage that is used for Z-stacks. This allows stage feedback to dictate when the sample is ready for an image. In our configuration, the X hardware stage corresponds to the Z stage within the software. This can be changed to match your stage setup by editing the following part of the configuration file: .. collapse:: Configuration File .. code-block:: yaml microscopes: microscope_name: stage: hardware: - type: ASI serial_number: 001 axes: [x, y, z, f] # software stages axes_mapping: [X, Y, Z, F] # hardware stages