======== Shutters ======== When only controlled with analog modulation, many laser sources do not reduce their emitted intensity to 0 watts. In such cases, residual illumination subjects the specimen to unnecessary irradiation between image acquisitions. Shutters overcome this by completely blocking the laser, albeit on a much slower timescale than direct modulation of the laser. With **navigate**, shutters automatically open at the start of acquisition and close upon finish. Most shutters are controlled via a digital voltage. When the voltage is high (e.g., > 2.5 V), the shutter is open, and when the voltage is low (e.g., < 2.5 V), the shutter is closed. ------------ National Instruments (NI) ---------------------------- We can control these shutters using a digital output from a National Instruments (NI) data acquisition card. .. Note:: If the shutter opens and closes immediately upon starting an acquisition, try a different port for the digital I/O on the NI data acquisition card. Some NI devices (e.g.PCIe-6738) have port/sample size limitations. If the port/sample size is exceeded, the shutter will not open. For example, on our `NI PCIe-6738 `_ using port 0 for the shutter causes this issue, but switching the shutter to any port 1 channel fixed it. In comparison, for the `NI PCIe-6259 `_, using port 0 had no averse effects. .. collapse:: Configuration File .. code-block:: yaml microscopes: microscope_name: shutter: hardware: type: NI channel: PXI6249/port0/line0 min: 0.0 max: 5.0 | ------------------ Synthetic Shutter ----------------- If no shutter is present, one must configure the software to use a synthetic shutter. .. collapse:: Configuration File .. code-block:: yaml microscopes: microscope_name: shutter: hardware: type: synthetic channel: PXI6249/port0/line0 min: 0.0 max: 5.0 |