COMPASS

Introduction

Why Light-Sheet Microscopy?
Light-Sheet Microscopy Optical Principles
NanoSPIM
ASLM
OPM

Design Principles

Required Software and Packages
Compass V1 General Design Process

Hardware Considerations

Preparation of the Imaging Room
Optical Table
Computer Specifications
Light Sources

Physical Assembly

Physical Assembly
Hardware
Parts List

Imaging with the System

Imaging Process
Image Stack Processing

System Characterization

System Characterization

COMPASS

System Characterization
View page source

System Characterization

Beam Characterization

Comparison of the expected light sheet beam from simulation to the experimentally imaged light sheet

Bead PSF Analysis

Analysis of the PSF of 100 nm beads in each direction

Sample Biological Images

Previous

© Copyright 2025, Dean Lab, UT Southwestern Medical Center.