Protocols

This section collects public Dean Lab protocols as a categorized library. The pages below normalize the source documents into a shared web format while preserving the values, units, and step order from the original protocols.

Cloning and Mutagenesis

• Gateway Cloning

• DNA Shuffling

• Error Prone PCR

• Saturated Mutagenesis

• Colony PCR

Bacterial Methods

• Preparation of Chemically Competent Cells

• Bacterial Electroporation

• Bacterial Colony Fluorescence

• Bacterial FACS

Protein and DNA Cleanup

• Protein Solubility

• GST Protein Purification

• Purification of Small DNA Fragments

• Ethanol Precipitation

Tissue Preparation and Clearing

• Labeling Tissue Samples

• BABB 2.0 Protocol

• Agarose Cube for Cleared Samples

Viral Production

• Retrovirus Production

• Viral Production - IMCD Cells

Imaging and Calibration

• Coating Beads on Coverslip

• Quantum Yield Protocol

Recipes

• Recipes