navigate.controller.sub_controllers.autofocus.AutofocusPopupController

class navigate.controller.sub_controllers.autofocus.AutofocusPopupController(view: AutofocusPopup, parent_controller: Controller)

Bases: GUIController

Class creates the popup to configure autofocus parameters.

__init__(view: AutofocusPopup, parent_controller: Controller) → None

Parameters:

• view (AutofocusPopup) – The view of the autofocus popup.

• parent_controller (navigate.controller.controller.Controller) – The parent controller of the autofocus popup.

Methods

__init__(view, parent_controller)	param view: The view of the autofocus popup.
close_popup(*_)	Close the popup window
display_plot(data_and_flags)	Display autofocus data, handling segmentation, plot mode, and redraw.
execute(command, *args)	This function is called when a command is passed from a child process.
handle_autofocus_complete(payload)	Handle autofocus completion metadata for defocus calibration.
initialize()	This function is called when the controller is initialized
populate_experiment_values()	Populate Experiment Values
register_event_listener(event_name, ...)	Register event listener in the parent_controller
set_acquisition_state(state)	Update the acquisition lifecycle state and popup actions.
set_autofocus_state(is_active)	Update autofocus activity and popup actions.
set_experiment_values()	Sets values of widgets based on experiment setting
show_autofocus_setting(*_)	Show Autofocus Parameters
show_verbose_info(*info)	Prints verbose information to the console
showup()	Shows the popup window
start_autofocus()	Starts the autofocus process.
stop_acquisition()	Stop the active acquisition through the main controller.
update_device_ref(*_)	Update device reference name
update_experiment_values(*args)	Collects all the values of widgets
update_setting_dict(parameter)	Show Autofocus Parameters

Attributes

CALIBRATION_ACTIONS
custom_events	Custom events for this controller
widgets	The autofocus setting dictionary.
microscope_name	The microscope name.
setting_dict	The autofocus setting dictionary.
autofocus_fig	The autofocus figure.
autofocus_coarse	The autofocus coarse plot.
defocus_calibration_reference	Temporary reference focus for defocus calibration.
coarse_plot	The autofocus coarse plot.

close_popup(*_: tuple[str]) → None

Close the popup window

Parameters:

_ (tuple[str]) – The event arguments.

display_plot(data_and_flags: tuple[numpy.ndarray, bool, bool]) → None

Display autofocus data, handling segmentation, plot mode, and redraw.

This method unpacks and normalizes incoming data, splits it into coarse and fine segments according to the current autofocus settings, renders scatter and/or line plots on the coarse axes, optionally clears previous data, marks the detected maximum(s), and schedules a non-blocking canvas redraw.

Parameters:

data_and_flags (tuple[np.ndarray, bool, bool]) – Tuple of (data, line_plot, clear_data). data contains sample rows with stage positions in column 0 and signal values in column 1. line_plot overlays a continuous red line when true, and clear_data clears the axes and draws peak indicators when true.

Raises:

ValueError – May be raised if data is empty or has fewer than two columns (propagated from internal processing or from _plot_maxima).

execute(command, *args)

This function is called when a command is passed from a child process.

Parameters:

• command (str) – command name

• args (tuple) – command arguments

handle_autofocus_complete(payload: dict) → None

Handle autofocus completion metadata for defocus calibration.

initialize()

This function is called when the controller is initialized

This function initializes GUI based on configuration setting parameter: configuration_controller set range value for entry or spinbox widgets; add values to combobox get other necessary information for configuration.yml

populate_experiment_values() → None

Populate Experiment Values

Populates the experiment values from the experiment settings dictionary

register_event_listener(event_name, event_handler)

Register event listener in the parent_controller

Parameters:

• event_name (str) – event name

• event_handler (function) – event handler

set_acquisition_state(state: str) → None

Update the acquisition lifecycle state and popup actions.

set_autofocus_state(is_active: bool) → None

Update autofocus activity and popup actions.

set_experiment_values()

Sets values of widgets based on experiment setting

setting_dict is a dictionary

show_autofocus_setting(*_: tuple[str]) → None

Show Autofocus Parameters

Parameters:

_ (tuple[str]) – The event arguments.

show_verbose_info(*info)

Prints verbose information to the console

Parameters:

info (tuple) – information to be printed

showup() → None

Shows the popup window

start_autofocus() → None

Starts the autofocus process.

stop_acquisition() → None

Stop the active acquisition through the main controller.

update_device_ref(*_: tuple[str]) → None

Update device reference name

Parameters:

_ (tuple[str]) – The event arguments.

update_experiment_values(*args)

Collects all the values of widgets

setting_dict is a reference of experiment dictionary update the dictionary directly

update_setting_dict(parameter: str) → callable

Show Autofocus Parameters

Parameters:

parameter (str) – The parameter to be updated.

Returns:

The function to update the parameter

Return type:

callable

autofocus_coarse

The autofocus coarse plot.

Type:

object

autofocus_fig

The autofocus figure.

Type:

object

coarse_plot

The autofocus coarse plot.

Type:

object

property custom_events: dict[str, callable]

Custom events for this controller

Returns:

The custom events for this controller.

Return type:

dict[str, callable]

defocus_calibration_reference

Temporary reference focus for defocus calibration.

Type:

dict

microscope_name

The microscope name.

Type:

str

parent_controller

parent controller

Type:

Controller

setting_dict

The autofocus setting dictionary.

Type:

dict

view

GUI view

Type:

tkinter.Tk

widgets

The autofocus setting dictionary.

Type:

dict