Dichroic Turrets

Dichroics can be used to reflect the excitation light towards the camera, while permitting the emission light that is captured by the objective to be transmitted to the camera. Alternatively, they can be placed in the detection path to separate different emission wavelengths and to direct it to the appropriate camera.

Currently, navigate incorporates a dichroic turret as a filter wheel, which enables you to add as many different dichroic turrets as you would like.

Note

The name parameter under hardware is optional. If not provided, the name of the device will be default to Filter 0, Filter 1, … Filter N in the GUI. However, if is is provided, then the GUI will automatically use the name provided as a label.

ASI

C60-CUBE-SLDR

The C60-CUBE-SLDR is a motorized dichroic slider that can be controlled by the ASI Tiger Controller. The example below shows the configuration file with one dichroic turret, and one filter wheel. The same communication instance is used for both the stage and filter wheel. It holds a maximum of 4 dichroics.

Configuration File

microscopes:
  microscope_name:
    -
      filter_wheel:
        hardware:
          type: ASI
          name: Filter Wheel 1
          wheel_number: 1
          port: COM1
          baudrate: 115200
        filter_wheel_delay: 0.03
        available_filters:
          Empty-Alignment: 0
          GFP: 1
          RFP: 2
          Far-Red: 3
    -
      filter_wheel:
        hardware:
          type: ASICubeSlider
          name: Dichroic Turret 1
          wheel_number: 2
          port: COM1
          baudrate: 115200
        filter_wheel_delay: 0.25
        available_filters:
          510LP: 0
          560LP: 1
          600LP: 2
          660LP: 3